Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus
Identifieur interne : 000A05 ( Main/Exploration ); précédent : 000A04; suivant : 000A06Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus
Auteurs : Kazuya Shirato [Japon] ; Shohei Semba [Japon] ; Sherif A. El-Kafrawy [Arabie saoudite] ; Ahmed M. Hassan [Arabie saoudite] ; Ahmed M. Tolah [Arabie saoudite] ; Ikuyo Takayama [Japon] ; Tsutomu Kageyama [Japon] ; Tsugunori Notomi [Japon] ; Wataru Kamitani [Japon] ; Shutoku Matsuyama [Japon] ; Esam Ibraheem Azhar [Arabie saoudite]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2018.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Animaux, Arabie saoudite, Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Fluorescence, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (médecine vétérinaire), Sensibilité et spécificité, Sondes oligonucléotidiques (génétique), Techniques d'amplification d'acides nucléiques (), Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Amorces ADN, Coronavirus du syndrome respiratoire du Moyen-Orient, Sondes oligonucléotidiques.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- Animaux, Arabie saoudite, Chameaux, Fluorescence, Humains, Sensibilité et spécificité, Techniques d'amplification d'acides nucléiques, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Animals, Camelus, Coronavirus Infections (diagnosis), Coronavirus Infections (veterinary), DNA Primers (genetics), Fluorescence, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Nucleic Acid Amplification Techniques (methods), Oligonucleotide Probes (genetics), Saudi Arabia, Sensitivity and Specificity.
- MESH :
- chemical , genetics : DNA Primers, Oligonucleotide Probes.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques.
- veterinary : Coronavirus Infections.
- Animals, Camelus, Fluorescence, Humans, Sensitivity and Specificity.
Abstract
Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed. Quenching probe (QProbe) can solve the problem in turbidity monitoring mechanism. Only primer-derived signal can be monitored specifically by QProbes. Two primer sets were developed to enable to confirm MERS case by RT-LAMP only. Both sets were highly specific and sensitive in comparison with real-time RT-PCR.
Url:
DOI: 10.1016/j.jviromet.2018.05.006
PubMed: 29763640
PubMed Central: 7113683
Affiliations:
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Le document en format XML
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first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Semba, Shohei" sort="Semba, Shohei" uniqKey="Semba S" first="Shohei" last="Semba">Shohei Semba</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Eiken Chemical Co., Ltd., 4-19-9 Taito, Taito-ku, Tokyo 110-8408, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Eiken Chemical Co., Ltd., 4-19-9 Taito, Taito-ku, Tokyo 110-8408</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="El Kafrawy, Sherif A" sort="El Kafrawy, Sherif A" uniqKey="El Kafrawy S" first="Sherif A." last="El-Kafrawy">Sherif A. El-Kafrawy</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589</wicri:regionArea><wicri:noRegion>Jeddah 21589</wicri:noRegion></affiliation></author><author><name sortKey="Hassan, Ahmed M" sort="Hassan, Ahmed M" uniqKey="Hassan A" first="Ahmed M." last="Hassan">Ahmed M. Hassan</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589</wicri:regionArea><wicri:noRegion>Jeddah 21589</wicri:noRegion></affiliation></author><author><name sortKey="Tolah, Ahmed M" sort="Tolah, Ahmed M" uniqKey="Tolah A" first="Ahmed M." last="Tolah">Ahmed M. Tolah</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589</wicri:regionArea><wicri:noRegion>Jeddah 21589</wicri:noRegion></affiliation></author><author><name sortKey="Takayama, Ikuyo" sort="Takayama, Ikuyo" uniqKey="Takayama I" first="Ikuyo" last="Takayama">Ikuyo Takayama</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Influenza virus Research Center, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza virus Research Center, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Influenza virus Research Center, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza virus Research Center, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Notomi, Tsugunori" sort="Notomi, Tsugunori" uniqKey="Notomi T" first="Tsugunori" last="Notomi">Tsugunori Notomi</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Eiken Chemical Co., Ltd., 4-19-9 Taito, Taito-ku, Tokyo 110-8408, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Eiken Chemical Co., Ltd., 4-19-9 Taito, Taito-ku, Tokyo 110-8408</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kamitani, Wataru" sort="Kamitani, Wataru" uniqKey="Kamitani W" first="Wataru" last="Kamitani">Wataru Kamitani</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Laboratory of Clinical Research on Infectious Diseases, Department of Pathogen Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Clinical Research on Infectious Diseases, Department of Pathogen Molecular Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871</wicri:regionArea><wicri:noRegion>Osaka 565-0871</wicri:noRegion></affiliation></author><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Acute Respiratory Viral Diseases and Cytokines, Department of Virology III, National Institute of Infectious Disease, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Azhar, Esam Ibraheem" sort="Azhar, Esam Ibraheem" uniqKey="Azhar E" first="Esam Ibraheem" last="Azhar">Esam Ibraheem Azhar</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589</wicri:regionArea><wicri:noRegion>Jeddah 21589</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589</wicri:regionArea><wicri:noRegion>Jeddah 21589</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Virological 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saoudite</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Fluorescence</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Sensibilité et spécificité</term><term>Sondes oligonucléotidiques (génétique)</term><term>Techniques d'amplification d'acides nucléiques ()</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>Oligonucleotide Probes</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Sondes oligonucléotidiques</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Fluorescence</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Arabie saoudite</term><term>Chameaux</term><term>Fluorescence</term><term>Humains</term><term>Sensibilité et spécificité</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques de diagnostic moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">Quenching probe (QProbe) can solve the problem in turbidity monitoring mechanism.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">Only primer-derived signal can be monitored specifically by QProbes.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">Two primer sets were developed to enable to confirm MERS case by RT-LAMP only.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">Both sets were highly specific and sensitive in comparison with real-time RT-PCR.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Adhikari, B R" uniqKey="Adhikari B">B.R. 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Nakayama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wahed, A A E" uniqKey="Wahed A">A.A.E. Wahed</name></author><author><name sortKey="Patel, P" uniqKey="Patel P">P. Patel</name></author><author><name sortKey="Heidenreich, D" uniqKey="Heidenreich D">D. Heidenreich</name></author><author><name sortKey="Hufert, F T" uniqKey="Hufert F">F.T. Hufert</name></author><author><name sortKey="Weidmann, M" uniqKey="Weidmann M">M. Weidmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L X" uniqKey="Wang L">L.X. Wang</name></author><author><name sortKey="He, L" uniqKey="He L">L. He</name></author><author><name sortKey="Fang, R" uniqKey="Fang R">R. Fang</name></author><author><name sortKey="Song, Q Q" uniqKey="Song Q">Q.Q. Song</name></author><author><name sortKey="Tu, P" uniqKey="Tu P">P. Tu</name></author><author><name sortKey="Jenkins, A" uniqKey="Jenkins A">A. Jenkins</name></author><author><name sortKey="Zhou, Y Q" uniqKey="Zhou Y">Y.Q. Zhou</name></author><author><name sortKey="Zhao, J L" uniqKey="Zhao J">J.L. Zhao</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name></region><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name><name sortKey="Kamitani, Wataru" sort="Kamitani, Wataru" uniqKey="Kamitani W" first="Wataru" last="Kamitani">Wataru Kamitani</name><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><name sortKey="Notomi, Tsugunori" sort="Notomi, Tsugunori" uniqKey="Notomi T" first="Tsugunori" last="Notomi">Tsugunori Notomi</name><name sortKey="Semba, Shohei" sort="Semba, Shohei" uniqKey="Semba S" first="Shohei" last="Semba">Shohei Semba</name><name sortKey="Takayama, Ikuyo" sort="Takayama, Ikuyo" uniqKey="Takayama I" first="Ikuyo" last="Takayama">Ikuyo Takayama</name></country><country name="Arabie saoudite"><noRegion><name sortKey="El Kafrawy, Sherif A" sort="El Kafrawy, Sherif A" uniqKey="El Kafrawy S" first="Sherif A." last="El-Kafrawy">Sherif A. El-Kafrawy</name></noRegion><name sortKey="Azhar, Esam Ibraheem" sort="Azhar, Esam Ibraheem" uniqKey="Azhar E" first="Esam Ibraheem" last="Azhar">Esam Ibraheem Azhar</name><name sortKey="Azhar, Esam Ibraheem" sort="Azhar, Esam Ibraheem" uniqKey="Azhar E" first="Esam Ibraheem" last="Azhar">Esam Ibraheem Azhar</name><name sortKey="Hassan, Ahmed M" sort="Hassan, Ahmed M" uniqKey="Hassan A" first="Ahmed M." last="Hassan">Ahmed M. Hassan</name><name sortKey="Tolah, Ahmed M" sort="Tolah, Ahmed M" uniqKey="Tolah A" first="Ahmed M." last="Tolah">Ahmed M. Tolah</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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